Refinement of Cost Reduction Technology in Sugarcane Micro propagation (2025)

Comparative Studies on Field Performance of Micropropagated and Conventionally Propagated Sugarcane Plants

Plant Tissue Culture and Biotechnology, 2008

The tissue culture derived sugarcane var. CoJ 64 plants attained better height, millable cane height and a greater number of live buds to conventionally raised plants. Further, the plants raised in vitro showed 13.2% increase in cane yield and 11.03% sugar recovery as compared to conventionally propagated sugarcane under parallel agronomic practices in the field, advocating suitability of seed cane programmed through tissue culture.Key words: Comparative studies, Field performance, Sugarcane, MicropropagationDOI = 10.3329/ptcb.v16i1.1102Plant Tissue Cult. & Biotech. 16(1): 25-29, 2006 (June)

PTC&B Comparative Studies on Field Performance of Micropropagated and Conventionally Propagated Sugarcane Plants

2015

The tissue culture derived sugarcane var. CoJ 64 plants attained better height, millable cane height and a greater number of live buds to conventionally raised plants. Further, the plants raised in vitro showed 13.2 % increase in cane yield and 11.03 % sugar recovery as compared to conventionally propagated sugarcane under parallel agronomic practices in the field, advocating suitability of seed cane programmed through tissue culture.

High throughput in vitro micropropagation of sugarcane (Saccharum officinarum L.) from spindle leaf roll segments: Cost analysis for agri-business industry

Plant Cell, Tissue and Organ Culture (PCTOC), 2014

High throughput micropropagation (HTM) protocol in sugarcane through direct shoot regeneration comprising five stages, was developed for agri-business industry. The distinction of the protocol lies in direct adventitious shoot regeneration without an intervening callus phase and liberty over the number of subculture passages leading to high rates of synchronous plant production. Stage 0 dealt with selection and maintenance of field-grown stock plants; Stage I marked the initiation of in vitro propagation on culture initiation medium using spindle leaf roll segments from apical tops; Stage II involved five passages (of 14 days each) on shoot multiplication medium followed by one additional passage (of 10 days) on shoot multiplication-cum-elongation medium. The shoot multiplication rates ranged from 4 to 25-fold in CoPb 91 and CoJ 83 cultivars, respectively; Stage III involved in vitro rooting on root induction medium and hardening for 14 days at each step; Stage IV comprised separation of clumps into single plantlets and transfer to soil. The complete plants were produced in 157 days with 97 percent survival rate. The fidelity of the protocol for agri-business industry was tested by producing approximately 0.1 million saleable HTM sugarcane plants in a small scale (150 m 2) tissue culture unit. The recurring (energy, labour, media, culture container, transplanting material) and non-recurring (laboratory infrastructure, glasshouse, equipment) parameters accounted for 75.56 and 24.44 percent production costs, respectively. The lowcost options for media, culture containers and transplanting material were incorporated and cost per plant was calculated to be US$ 0.13. The HTM protocol was adopted by two sugar mills, and the HTM plants produced in our tissue culture unit were sold for seed multiplication to eight sugar mills and 430 farmers throughout North India. Keywords Agri-business industry Á Cost analysis Á Direct shoot regeneration Á High throughput micropropagation Á Leaf roll segments Á Sugarcane Abbreviations BAP 6-Benzyl amino purine CIM Culture initiation medium Electronic supplementary material The online version of this article (

Improving Utilization of Sugarcane by Replacing Ordinary Propagation Material with Small Chips of Sugarcane Planted in Paper Pots

Zenodo (CERN European Organization for Nuclear Research), 2017

Sugarcane is an important resource for bioenergy. Fields are usually established by using 15-20 cm pieces of sugarcane stalks as propagation material. An alternative method is to use small chips with nodes from sugarcane stalks. Plants from nodes are often established in plastic pots, but plastic pots could be replaced with biodegradable paper pots. This would be a more sustainable solution, reducing labor costs and avoiding pollution with plastic. We compared the establishment of plants from nodes taken from three different part of the sugarcane plant. The nodes were planted in plastic and paper pots. There was no significant difference between plants established in the two pot types. Nodes from different part of the stalk had different sprouting capacity. Nodes from the top parts sprouted significantly better than nodes taken from the middle or nodes taken closed to the ground in two experiments. Nodes with a length of 3 cm performed better than nodes with a length of 2 cm.

Enhancing the Protocol for Efficient Sugarcane (Saccharumofficinarum) Micropropagation using the BioMINT Temporary Immersion System in the Variety B79-474

2018

Sugarcane (Saccharum spp.) is a cultivated crop in Belize which is used as food source and helps generate income especially for rural areas. The variety B79-474 is the most dominant due to its robustness. In this study, the BioMINT temporary immersion system (TIS) was used to enhance the protocol for efficient sugarcane micropropagation. Sugarcane plants were micropropagated using the BioMINT TIS to determine the appropriate subculture interval for the variety B79-474. The subculture intervals studied were 30, 45 and 60 days. After the time interval, parameters such as average plant height, survival rate, dry weight and multiplication coefficient were used to determine the appropriate subculture interval. The best results from the subculture intervals proved to be 45 days based on the parameters evaluated. Different volumes of culture media were also tested. These included 300 mL, 400 mL and 500 mL. In the parameters evaluated and most crucial in the multiplication phase, the best r...

In Vivo Propagation Responses of Sugarcane (Sacharum Officinarum L) Genotypes at Metahara Sugar Estate of EthiopiaLeaves

Journal of Applied Biotechnology & Bioengineering

In vivo propagation of micropropagated sugarcane plants of three selected sugarcane genotypes was carried out with the objective of evaluating their proliferation responses to BAP. Accordingly, six levels of BAP (0, 0.5, 1, 1.5, 2 and 2.5mgL-1) with three sugarcane genotypes in a completely randomized design with 6×3 factorial treatment combination arrangements were tested. Data on the number of tillers per shoot, average shoot length (cm) and number of leaves per shoot were collected after 60 days of planting on white polyethylene bag having 10 cm height and 8 cm diameter filled with soil, compost and sand at the ratios of 8:3:2 respectively. Analysis of variance revealed that the interaction effects of BAP and the sugarcane genotypes was very highly significant (P<0.0001) on the response variables tested. In sugarcane genotype C123-81, the optimum number of tillers per shoot (4.00) and average shoot length (30.67cm) were recorded on 1mgL-1 BAP while the maximum number of leaves (6.00) was obtained at 0.5 and 1mgL-1. In sugarcane genotype C86-156, the optimum number of tillers per shoot (3.53) and number of leaves per shoot (7.00) were obtained at 1.5mgL-1 BAP while the maximum average shoot length was observed at 1mgL-1. In sugarcane genotype SP70-1284, the maximum average shoot length (32.67cm) and highest number of leaves per shoot (6.53) were obtained at 1mgL-1 BAP while the optimum number of tillers per shoot (4.67) was recorded at 1mgL-1 BAP. Therefore, from this result it can be concluded that, using this result, on average a plantlet can produce 4 tillers per shoot within a month and can reduce plantlets procurement cost by 25%.

Field performance of micropropagated plants and potential of seed cane for stalk yield and quality in sugarcane

Sugar Tech, 2009

Two field experiments were conducted to ascertain the potential of micropropagation technique for faster production of seed cane by using tissue culture plants raised through apical meristem culture in first generation (TC 0) followed by clonal propagation through cane setts in next generation (TC1). About 18, 520 plants, produced from a single shoot through micropropagation, were required at row to row and plant to plant spacing of 90 and 60 cm, respectively as compared to 88 quintal of cane seed in conventional methods for planting in an area of one hectare. Multiplication ratio was 100-150 times using tissue culture plants as compared to 11-12 using conventional cane setts, leading to drastic reduction in seed cane requirement. The TC 1 exhibited superiority over vegetatively propagated conventional crop for millable canes and stalk yield by 17 and 10.4 per cent, respectively. Though the single cane weight and cane diameter (non-significantly) were slightly lesser in TC1 as compared to conventional crop, this did not distress its potential as seed crop. The incidence of Ratoon Stunting Disease (RSD) and Leaf Scald Disease (LSD) was very low in TC 1 crop as compared to conventional crop. The findings established the potential of tissue culture technique for the production of quality seed free of pests and pathogens in the existing varieties and rapid multiplication of newly released varieties for quick adoption by the growers.

Research article -Sugarcane

Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution of diseases. Micropropagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material. Experiments on shoot tip culture initiation and shoot multiplication were laid out in completely randomized design with 2x3x3 and 4x5x3 factorial treatment arrangements respectively. Data was subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. With regard to shoot multiplication, genotype Q200 showed a maximum of 13.59 shoots per explant with 5.83cm shoot length on a medium fortified with 2 mg/l BAP alone, while genotype Q217 produced a maximum of 15.28 shoots per explant with 5.37cm mean shoot length on a medium supplied with 2.0 mg/l BAP and 0.25 mg/l kinetin. Likewise, Co-0238 produced maximum of 13.56 shoots per explant with mean shoot length 6.50 cm on medium fortified with 1.5 mg/l BAP + 0.5mg/l kinetin

Bud chip method of sugarcane planting: A review M Mohanty and PK Nayak

Sugarcane crop requires huge quantity of seed cane for planting under conventional method, which contributes a major share in cost of cultivation. Besides, large quantity of seed material poses a big challenge for transportation and handling. This problem can be effectively addressed through adoption of sustainable sugarcane initiative through planting of bud chips, which can save the cost and inconveniences associated with conventional planting methods. Several authors have reported advantages of planting single bud chips over conventional methods with respect to germination, crop establishment, growth and development of sugarcane crop. Sustainable sugarcane initiative technology favourably influenced various yield attributing factors such as plant stand, millable cane per clump and weight of single cane thereby resulting in higher yield. Some authors have also recorded higher brix value and higher juice weight at harvesting stage with planting of single chip bud seedlings of sugarcane. Compared with conventional method, economics of cultivation goes in favour of bud chip method of planting. Based on research findings by various workers, it can be said that planting of sugarcane by bud chip method is superior to planting by conventional methods. Introduction Sugarcane (Saccharum officinarum L.) occupies an important position among commercial crops grown in the world. This crop is efficient in utilizing solar energy for production of sugar and other renewable energy (Mohanty et al., 2015) [14]. Sugarcane cultivation is facing several challenges due to increasing cost of input and labor (Loganandhan et al., 2013) [11]. Under conventional method, planting material occupies a major chunk in cost involved in sugarcane cultivation. Depending on variety and method of growing, there is need for huge quantity of seed cane for planting of sugarcane. Requirement of huge quantity of seed material also poses a big challenge for transportation, handling and planting (Kumar, 2020) [10]. Srivastava et al. (1981) [24] also mentioned that a large quantity (6-8 t/ha) of 3-budded setts are required for sugarcane planting, which is nearly 22 to 25 % of the total cost of production. In order to reduce the overall cost of production and the drudgery involved in handling huge quantity of planting materials, there is strong need to develop suitable technology for sugarcane cultivation. To address this situation, many authors have suggested adoption of sustainable sugarcane initiative (SSI) with Bud Chip Technology, which can save large quantities of seed canes (Loganandhan et al., 2013; Parajuli et al., 2019) [11, 18]. Under farmers' field situation, Mishra (2019) [12] suggested use of axillary buds of sugarcane plant, generally known as bud chips, for reducing the volume of seed material and augmenting the quality of seed cane. In this method, a root primordium along with small volume of tissue adhering to the bud is used for regeneration of sugarcane plant. Bud chip method of sugarcane growing can save nearly 80% of the stalk material used for planting (Jain et al., 2010) [5, 6] that can be alternatively used for consumption purpose. Arthi et al. (2016) [1] opined that sustainable Sugarcane Initiative (SSI) is a new method, which can boost the productivity of sugarcane by utilizing less resource such as seed, water and space. Shanthy and Ramanjaneyulu (2014) [23] described Sustainable Sugarcane Initiative (SSI) as a combination of many viable technologies in order to enhance the yield of sugarcane. Sugarcane production under sustainable sugarcane initiative technique minimizes the requirement of seed & water and enables the crop for proper utilization of plant nutrients to obtain higher yield (Loganandhan et al., 2013; Naik et al,. 2015) [11, 16]. As per Parajuli et al. (2019) [18] , Sustainable Sugarcane Initiative aims at providing valuable solutions to the farmers for enhancement of productivity of land, water and human labour.

DEVELOPMENT OF PROTOCOL FOR MASS MULTIPLICATION OF TWO ELITE VARIETIES OF SUGARCANE THROUGH MICROPROPAGATION.

Sugarcane (Saccharum officinarum L.) also called "noble canes" by Dutch scientists, belongs to family Poaceae and genus Saccharum. Sugar cane is the main source of sugar in all tropical and subtropical countries of the world with an annual production of 40 - 41 million tons. Sugar i.e. sucrose is necessary part of daily diet and a major portion of total sugar produced is used in alcoholic beverages, soft drinks, ice-creams, chocolates, canning industries and its by-products i.e. molasses and bagasse are also utilized as raw material for manure, fiber board and paper making units and even as fuel in sugar mills. Sugar cane is a tall, perennial grasses which is propagated vegetatively by stem cuttings called ‘setts’ having healthy buds. The conventionally propagated setts thus use a substantial number of canes and yet do not ensure uniformity, pest free or disease free status of planting material. The in vitro regeneration methods instead ensure large number of uniform, disease free and vigorous planting material in relatively lesser time. I this study we were emphases on the shoot tips containing axillary meristem were inoculated in MS medium with a fixed concentration of BAP (1mg/lit) for shoot tip initiation and establishment. Small shoots started appearing with in 7-10 days in all cultures bottles. Maximum shoot lengths were obtained in SM-II media i.e. MS supplemented with 0.25mg/lit BAP. After 30 days of incubation the average shoot length recorded in SM-II medium was found to be 1.63cm in Co-86032 and 1.65cm in C0-94012. Similarly the regenerated shoots were used for root induction in root forming media. The response of root formation was different in all media, as highest root formation average was observed in RM-III (3 mg/lit NAA & 3 mg/lit IBA) followed by RM-II media. Average number of plants showing roots was 9.6 in Co-86032 whereas it was recorded low in Co-94012 i.e. 7.8 in RM-III.